Publicaties

Gene therapy is a field of research in which a huge amount of effort has been spent over the last two decades. To succeed, a correct therapeutic gene needs to be delivered into the cytoplasm of a cell for mRNA and nucleus of a cell for plasmid DNA (pDNA). To protect the nucleic acids, viral vectors and non-viral carriers have been used. Due to the many barriers that are encountered in the delivery, the design and evaluation of especially the ...

In the last two decades, fluorescent proteins became an indispensable tool to noninvasively label a protein in living cells. The discovery of photoswitchable fluorescent proteins expanded the applications of the fluorescent proteins to techniques such as molecular tracking and highlighting on a microscope. Recently, a new microscopic modality to achieve a superresolution circumventing the diffraction limit of light (photoactivated localization ...

In the last two decades, fluorescent proteins became an indispensable tool to noninvasively label a protein in living cells. The discovery of photoswitchable fluorescent proteins expanded the applications of the fluorescent proteins to techniques such as molecular tracking and highlighting on a microscope. Recently, a new microscopic modality to achieve a superresolution circumventing the diffraction limit of light (photoactivated localization ...

The TIE2 receptor (tyrosine kinase with immunoglobulin-like and epidermal growth factor homology domains-2), a member of the tyrosine kinase receptor family, was initially discovered and studied in endothelial cells. Recent literature shows that this protein is also present on, but not exclusively, pericytes, macrophages or various cancer lines. By using immunogold labeling, Western Blot and qPCR (quantitative polymerase chain reaction), our ...

Fluorescence microscopy, arguably the most popular branch of optical microscopy, has undergone tremendous changes in recent years. These include ultrasensitive detection down to single molecules and the development of schemes that circumvent the diffraction limit. This chapter gives some background to single-molecule fluorescence microscopy, the most popular microscopy modes for single-molecule detection and a selection of polymer problems that ...

Dealuminated zeolites are widely used acid catalysts in research and the chemical industry. Bulk-level studies have revealed that the improved catalytic performance results from an enhanced molecular transport as well as from changes in the active sites. However, fully exploiting this information in rational catalyst design still requires insight in the intricate interplay between both. Here we introduce fluorescence and stimulated Raman ...

The data provided with this paper are image series of slowly diffusing GlyRa3 molecules, linked to either eGFP or mCherry fluorescent proteins, at the membrane of HEK cells, acquired on a Zeiss LSM880 confocal laser scanning microscope. Raster spectral image cross-correlation spectroscopy (RSICS) is applied to the data, a technique that exploits intensity fluctuations in confocal image series recorded using a spectral detector to study the ...