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Soft tissue and bone tumor diagnostics : harnessing the power of molecular techniques Universiteit Gent

Fleur Cordier, Liesbeth Ferdinande, Anne Hoorens, Koen Van de Vijver, Jo Van Dorpe, David Creytens
Since the introduction of new molecular techniques, the diagnostic landscape of soft tissue and bone tumors has expanded greatly over the past few years. The use of new molecular techniques has led to the identification of new genetic alterations and, therefore, to a better understanding of tumorigenesis, tumor detection and classification. Furthermore, methylation profiling has emerged as a classification tool for soft tissue and bone tumors. ...

Performance of parasitological and molecular techniques for the diagnosis and surveillance of gambiense sleeping sickness Instituut voor Tropische Geneeskunde

Dieudonné Mumba-Ngoyi, Rosine Ali Ekangu, Marie France Mumvemba Kodi, Patient Pyana Pati, Fatima Balharbi, Mélanie Decq, Victor Kande Betu, Wim Van der Veken, Claude Sese, Joris Menten, et al.

OBJECTIVES: Recently, improvements have been made to diagnostics for gambiense sleeping sickness control but their performance remains poorly documented and may depend on specimen processing prior to examination. In a prospective study in the Democratic Republic of the Congo, we compared the diagnostic performance of several parasite detection techniques, immune trypanolysis and of m18S PCR on whole blood stored in a stabilisation buffer or ...

Novel techniques and future directions in molecular diagnosis of malaria in resource-limited settings Instituut voor Tropische Geneeskunde Universiteit Antwerpen KU Leuven

Cheryll Eniyou Oriero, Jean-Pierre Van Geertruyden, Davis C Nwakanma, D'Alessandro, Jan Jacobs

Despite being preventable and treatable, malaria remains a global health concern with approximately 1.2 billion people at high risk of being infected, 90% of whom are in the resource-limited settings of sub-Saharan Africa. The continued decline in malaria cases globally has rekindled the possibility of elimination in certain regions. As humans constitute the main reservoir of malaria, prompt and accurate diagnosis by microscopy or rapid ...

Knowledge of morphology is still required when identifying new amoeba isolates by molecular techniques Instituut voor Tropische Geneeskunde

J.F. De Jonckheere, S. Gryseels
We have isolated several free-living amoeba strains from the environment in Ghana, which have internal transcribed spacers, including the 5.8S rDNA, sequences similar to sequences attributed to Vahlkampfiidae (Heterolobosea) in databases. However, morphological examination shows that the isolates belong to the Hartmannellidae (Amoebozoa). We provide evidence that the sequences in the databases are wrongly classified as belonging to a genus or ...

Application of molecular techniques in the study of natural infection of Leishmania infantum vectors and utility of sandfly blood meal digestion for epidemiological surveys of leishmaniasis Instituut voor Tropische Geneeskunde

M.M. Alcover, M. Gramiccia, T. Di Muccio, C. Ballart, S. Castillejo, Albert Picado De Puig, M. Portús, M. Gállego
Epidemiological studies on the distribution of leishmaniasis caused by Leishmania infantum Nicolle, 1908 (Kinetoplastida: Trypanosomatidae) have been based principally on serological surveys of the canine reservoir. This methodology is useful due to the facility of sampling, the rapidity in obtaining results, its consistency and because it allows the detection of heterogeneous foci of canine leishmaniasis (CanL) even in small areas. Other ...

Diagnostic accuracy of molecular amplification tests for human african trypanosomiasis-systematic review Instituut voor Tropische Geneeskunde

CM Mugasa, ER Adams, KR Boer, HC Dyserinck, P Büscher, HD Schallig, MM Leeflang
BACKGROUND: A range of molecular amplification techniques have been developed for the diagnosis of Human African Trypanosomiasis (HAT); however, careful evaluation of these tests must precede implementation to ensure their high clinical accuracy. Here, we investigated the diagnostic accuracy of molecular amplification tests for HAT, the quality of articles and reasons for variation in accuracy. METHODOLOGY: Data from studies assessing diagnostic ...

Comparison of microsatellite instability detection by immunohistochemistry and molecular techniques in colorectal and endometrial cancer Universiteit Gent Vrije Universiteit Brussel

Franceska Dedeurwaerdere, Kathleen Claes, Jo Van Dorpe, Isabelle Rottiers, Joni Van der Meulen, Joke Breyne, Koen Swaerts, Geert Martens
DNA mismatch repair deficiency (dMMR) testing is crucial for diagnosing Lynch syndrome and detection of microsatellite unstable (MSI) tumors eligible for immunotherapy. The aim of this study was to compare the relative diagnostic performance of three molecular MSI assays: polymerase chain reaction (PCR), MSI testing by Idylla and next-generation-sequencing (NGS) on 49 tumor samples (28 colorectal and 21 endometrial adenocarcinomas) versus ...