Publicaties
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Confronting bias and precision in digital PCR quantification Universiteit Gent
Comparison of digital PCR platforms and semi-nested qPCR as a tool to determine the size of the HIV reservoir Universiteit Gent
Comparison of quantitative PCR and MALDI-TOF mass spectrometry assays for identification of bacteria in milk samples from cows with subclinical mastitis Universiteit Gent
The digital MIQE guidelines update : minimum information for publication of quantitative digital PCR experiments for 2020 Universiteit Gent
Digital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with advances in instrumentation, software, applications, and our understanding of its technological potential. Yet these developments also have associated challenges; data analysis steps, including threshold setting, can be difficult and preanalytical steps required to purify, ...
Development of bioinformatics tools for high-throughput PCR applications Universiteit Gent
This thesis is built around three chapters. The first introductory chapter gives an overview of the laboratory methods for which I developed bioinformatic tools. The basic principles of PCR are discussed, as well as the evolution towards quantitative (qPCR) and digital PCR (dPCR). Next, Sanger and so-called next-generation sequencing are discussed, ending with an overview of the current DNA target enrichment strategies. Chapter 2 entails four ...
IMPLANT : a new technique for transgene copy number estimation in plants using a single end-point PCR reaction Universiteit Gent Vlaams Instituut voor Biotechnologie
Background: Copy number determination is one of the first steps in the characterization of transgenic plant lines. The classical approach to this, Southern blotting, is time-consuming, expensive and requires massive amounts of high-quality genomic DNA. Other PCR-based techniques are either inaccurate, laborious, or expensive. Results: Here, we propose a new technique, IMPLANT (Insertion of competitive PCR calibrator for copy number estimation), ...