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Digital PCR as a tool to measure HIV persistence Universiteit Gent
Quality control of digital PCR assays and platforms Universiteit Gent
Quantification of integrated HIV DNA by repetitive-sampling Alu-HIV PCR on the basis of Poisson statistics Universiteit Gent
Rapid diagnostic tests as a source of DNA for Plasmodium species-specific real-time PCR Instituut voor Tropische Geneeskunde
BACKGROUND: This study describes the use of malaria rapid diagnostic tests (RDTs) as a source of DNA for Plasmodium species-specific real-time PCR. METHODS: First, the best method to recover DNA from RDTs was investigated and then the applicability of this DNA extraction method was assessed on 12 different RDT brands. Finally, two RDT brands (OptiMAL Rapid Malaria Test and SDFK60 malaria Ag Plasmodium falciparum/Pan test) were comprehensively ...
Giemsa-stained thick blood films as a source of DNA for Plasmodium species-specific real-time PCR Instituut voor Tropische Geneeskunde
BACKGROUND: This study describes the use of thick blood films (TBF) as specimens for DNA amplification with the Plasmodium species-specific real-time PCR that was recently validated on whole blood samples. METHODS: The panel of 135 Giemsa-stained clinical TBFs represented single infections of the four Plasmodium species with varying parasite densities or only gametocytes, mixed infections, and negative samples and was stored for up to 12 years. ...
Diagnostic accuracy of PCR in gambiense sleeping sickness diagnosis, staging and post-treatment follow-up: a 2-year longitudinal study Instituut voor Tropische Geneeskunde
BACKGROUND: The polymerase chain reaction (PCR) has been proposed for diagnosis, staging and post-treatment follow-up of sleeping sickness but no large-scale clinical evaluations of its diagnostic accuracy have taken place yet. METHODOLOGY/PRINCIPAL FINDINGS: An 18S ribosomal RNA gene targeting PCR was performed on blood and cerebrospinal fluid (CSF) of 360 T. brucei gambiense sleeping sickness patients and on blood of 129 endemic controls from ...
A Schistosoma haematobium-specific real-time PCR for diagnosis of urogenital schistosomiasis in serum samples of international travelers and migrants Instituut voor Tropische Geneeskunde
BACKGROUND: Diagnosis of urogenital schistosomiasis by microscopy and serological tests may be elusive in travelers due to low egg load and the absence of seroconversion upon arrival. There is need for a more sensitive diagnostic test. Therefore, we developed a real-time PCR targeting the Schistosoma haematobium-specific Dra1 sequence. METHODOLOGY/PRINCIPAL FINDINGS: The PCR was evaluated on urine (n = 111), stool (n = 84) and serum samples (n = ...
Validation of a four-primer real-time PCR as a diagnostic tool for single and mixed Plasmodium infections Instituut voor Tropische Geneeskunde
While microscopy remains the gold standard for malaria diagnosis, molecular tools gain more interest. To improve the detection of mixed-infections, we developed a four-primer real-time PCR with 4 Plasmodium species-specific forward primers, based on the pan-primer design with universal Plasmodium primers as described before. After validation for analytical sensitivity, specificity and reproducibility, the four-primer-PCR was evaluated on 351 ...