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Phylogenetic variation of Tri1 gene and development of PCR–RFLP analysis for the identification of NX genotypes in Fusarium graminearum species complex Universiteit Gent

Meiling Gao, Mengyuan Zhang, Jiahui Zhang, Xianli Yang, Mohamed Fathi Abdallah Abdelmohsen, Jianhua Wang

NX toxins have been described as a novel group of type A trichothecenes produced by members of the Fusarium graminearum species complex (FGSC). Differences in structure between NX toxins and the common type B trichothecenes arise from functional variation in the trichothecene biosynthetic enzyme Tri1 in the FGSC. The identified highly conserved changes in the Tri1 gene can be used to develop specific PCR-based assays to identify the NX-producing ...

Application of PCR-RFLP for the exploration of the molecular diversity of Leishmania infantum in Algeria Instituut voor Tropische Geneeskunde Universiteit Antwerpen

N Seridi, M Belkaid, Wilber Quispe, C Zidane, JC Dujardin

Ghibe river basin in Ethiopia: present situation of trypanocidal drug resistance in Trypanosoma congolense using tests in mice and PCR-RFLP Instituut voor Tropische Geneeskunde

Y. Moti, R Fikru, Jan Van Den Abbeele, Philippe Büscher, Peter Van den Bossche, L. Duchateau, Vincent Delespaux

Improved PCR-RFLP for the detection of diminazene resistance in Trypanosoma congolense under field conditions using filter papers for sample storage Instituut voor Tropische Geneeskunde

HS Vitouley, EO Mungube, E Allegye-Cudjoe, O Diall, Z Bocoum, B Diarra, TF Randolph, B Bauer, PH Clausen, Dirk Geysen, et al.

Development and validation of a PCR–RFLP test to identify African Rhipicephalus (Boophilus) ticks Instituut voor Tropische Geneeskunde

L. Lempereur, Dirk Geysen, Maxime Madder

PCR-RFLP y RAPD para la tipificación de Leishmania neotropical Instituut voor Tropische Geneeskunde

AM Montalvo, L Monzote, J. Fraga, I Montano, C Muskus, M Marín, Simonne De Doncker, ID Vélez, JC Dujardin

SSCP analysis of the P2 purine transporter TcoAT1 gene of Trypanosoma congolense leads to a simple PCR-RFLP test allowing the rapid identification of diminazene resistant stocks Instituut voor Tropische Geneeskunde

Vincent Delespaux, S. Chitanga, Dirk Geysen, A Goethals, Peter Van den Bossche, Stanny Geerts

PCR-RFLP using Ssu-rDNA amplification: applicability for the diagnosis of mixed infections with different trypanosome species in cattle Instituut voor Tropische Geneeskunde

Vincent Delespaux, F Ayral, Dirk Geysen, Stanny Geerts

Presence of Anopheles culicifacies B in Cambodia established by the PCR-RFLP assay developed for the identification of Anopheles minimus species A and C and four related species Instituut voor Tropische Geneeskunde

Wim Van Bortel, T. Sochantha, RE Harbach, D Socheat, Patricia Roelants, T Backeljau, Marc Coosemans

Comparative evaluation of the nested ITS PCR against the 18S PCR-RFLP in a survey of bovine trypanosomiasis in Kwale County, Kenya Vrije Universiteit Brussel Universiteit Gent

Steven Odongo, Vincent Delespaux, Maina Ngotho, Serkalem Bekkele, Stefan Magez

We compared the nested internal transcribed spacer (ITS) PCR and the 18S PCR RFLP (restriction-fragment length polymorphism) pan-trypanosome assays in a cross-sectional survey of bovine trypanosomiasis in 358 cattle in Kwale County, Kenya. The prevalence of trypanosomiasis as determined by the nested ITS PCR was 19.6% (70/358) and by 18S PCR-RFLP was 16.8% (60/358). Of the pathogenic trypanosomes detected, the prevalence of Trypanosoma ...

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