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The perceived impact of isoniazid resistance on outcome of first-line rifampicin-throughout regimens is largely due to missed rifampicin resistance Instituut voor Tropische Geneeskunde
Background: Meta-analyses on impact of isoniazid-resistant tuberculosis informed the World Health Organization recommendation of a levofloxacin-strengthened rifampicin-based regimen. We estimated the effect of initial rifampicin resistance (Rr) and/or isoniazid resistance (Hr) on treatment failure or relapse. We also determined the frequency of missed initial and acquired Rr to estimate the impact of true Hr.
Methods: Retrospective ...
Rifampicin drug resistance tests for tuberculosis: challenging the gold standard Instituut voor Tropische Geneeskunde
Setting:The rapid diagnosis of rifampicin resistance is hampered by reported insufficient specificity of molecular techniques for detection of rpoB mutations. Objective:To document the prevalence and prognostic value of rpoB mutations with unclear phenotypic resistance.Design:Sequencing directly from sputum of first failure or relapse patients without phenotypic selection; comparison of standard re-treatment regimen outcome by mutation. ...
Investigation of rifampicin resistance mechanisms in Brucella abortus using MS-driven comparative proteomics Universiteit Gent
Mutations in the rpoB gene have already been shown to contribute to rifampicin resistance in many bacterial strains including Brucella species. Resistance against this antibiotic easily occurs and resistant strains have already been detected in human samples. We here present the first research project that combines proteomic, genomic, and microbiological analysis to investigate rifampicin resistance in an in vitro developed rifampicin resistant ...
Rifampicin resistance missed in automated liquid culture system for Mycobacterium tuberculosis with specific rpoB-mutations Instituut voor Tropische Geneeskunde
WHO-endorsed phenotypic drug-susceptibility-testing (DST) methods for Mycobacterium tuberculosis are assumed to be the gold standard for identifying rifampicin (RMP) resistance. However, previous results indicate that low-level - yet probably clinically relevant - RMP resistance linked to specific rpoB mutations is easily missed by some growth-based methods. We aimed to compare the level of resistance on Lowenstein-Jensen (LJ) medium with ...