Publicaties

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Isolation of Candidatus rickettsia vini from Belgian Ixodes arboricola ticks and propagation in tick cell lines Instituut voor Tropische Geneeskunde Universiteit Hasselt Universiteit Antwerpen

Alaa M. Al-Khafaji, Lesley Bell-Sakyi, Gerardo Fracasso, Lisa Luu, Erik Matthysen, Jose A. Oteo, Ana M. Palomar, Dieter Heylen

Candidatus Rickettsia vini was originally detected in Ixodes arboricola ticks from Spain, and subsequently reported from several other Western Palearctic countries including Belgium. Recently, the bacterium was isolated in mammalian (Vero) cell culture from macerated male I. arboricola from Czech Republic, but there have been no reports of propagation in tick cells. Here we report isolation in a tick cell line of three strains of Ca. R. vini ...

Molecular screening of cattle ticks, tick-borne pathogens and amitraz resistance in ticks of Santo Domingo de los Ts & aacute;chilas province in Ecuador Instituut voor Tropische Geneeskunde

Alicia Maya-Delgado, Maxime Madder, Washington Benitez-Ortiz, Claude Saegerman, D. Berkvens, Lenin Ron-Garrido

The province of Santo Domingo de los Tsachilas in Ecuador is a strategic place for cattle production and trade. The lack of knowledge about tick species, tick control and tick-borne diseases in Ecuador prompted this study with the goal of identifying the cattle-tick species and tick-borne agents present in the area and molecularly determining the potential acaricide resistance to amitraz of the major cattle tick species. Eighty-four cattle ...

Managing the bricks and the clicks – Omnichannel retail logistics from an in-store staff perspective Vrije Universiteit Brussel

Heleen Buldeo Rai, Sara Verlinde, Lien Martien Bauwens, Cathy Macharis
The attention for omnichannel retail has increased impressively in the last years, making it a buzz-word in business and research. Articles on the topic found on the scientific database Google Scholar emerged as from 2012 and have since then almost doubled every year. Omnichannel retail entails a strategy that integrates online and offline retail channels and is generally implemented by pure retail players with only one channel distinctly ...

Identification of hard ticks (Acari: Ixodidae) and seroprevalence to Theileria parva in cattle raised in North Kivu Province, Democratic Republic of Congo Instituut voor Tropische Geneeskunde

M.K. Kalume, C Saegerman, D.K. Mbahikyavolo, A.M. Makumyaviri, T. Marcotty, Maxime Madder, Y. Caron, L. Lempereur, B Losson
This study aimed to identify tick species and to determine their relationship with the Theileria parva seroprevalence in cattle raised under an extensive farming system in North Kivu Province, Democratic Republic of Congo in two agro-ecological zones namely medium (1,000-1,850 m) and high (>1,850 m) altitude. Among the 3,215 ticks collected on 482 animals, from February to April 2009, Rhipicephalus appendiculatus (64.26 %), the main vector of ...

Pathogen communities of songbird-derived ticks in Europes low countries Universiteit Antwerpen

Dieter Heylen, Manoj Fonville, Arieke Docters van Leeuwen, Arjan Stroo, Martin Duisterwinkel, Sip van Wieren, Maria Diuk-Wasser, Arnout de Bruin, Hein Sprong
Background Birds play a major role in the maintenance of enzootic cycles of pathogens transmitted by ticks. Due to their mobility, they affect the spatial distribution and abundance of both ticks and pathogens. In the present study, we aim to identify members of a pathogen community [Borrelia burgdorferi (s.l.), B. miyamotoi, Ca. Neoehrlichia mikurensis, Anaplasma phagocytophilum and Rickettsia helvetica] in songbird-derived ticks from 11 ...

A transcriptome-based phylogenetic study of hard ticks (Ixodidae) Universiteit Antwerpen Universiteit Hasselt

N. Pierre Charrier, Axelle Hermouet, Caroline Hervet, Albert Agoulon, Stephen C. Barker, Dieter Heylen, Celine Toty, Karen D. McCoy, Olivier Plantard, Claude Rispe, et al.
Hard ticks are widely distributed across temperate regions, show strong variation in host associations, and are potential vectors of a diversity of medically important zoonoses, such as Lyme disease. To address unresolved issues with respect to the evolutionary relationships among certain species or genera, we produced novel RNA-Seq data sets for nine different Ixodes species. We combined this new data with 18 data sets obtained from public ...

Host identification in unfed ticks from stable isotope compositions ($\delta^{13}C$ and $\delta^{15}N$) Universiteit Antwerpen Universiteit Hasselt

Dieter Heylen, O. Schmidt, H. Dautel, L. Gern, H. Kampen, J. Newton, J. Gray
Determination of the ratios of natural stable isotopes (13C/12C and 15N/14N) in unfed Ixodes ricinus nymphs and adults, which, in their previous stage, fed on captive wild rodents (Apodemus sylvaticus and Myodes glareolus), wild birds (Parus major and Cyanistes caeruleus) or domestic ruminants (Ovis aries and Bos taurus), demonstrated that it is possible to identify each host category with confidence. First, the tickblood spacing, which is the ...