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The Trypanosoma cruzi satellite DNA OligoC-TesT and Trypanosoma cruzi kinetoplast DNA OligoC-TesT for diagnosis of Chagas disease Instituut voor Tropische Geneeskunde

Koen De Winne, Philippe Büscher, Alejandro O Luquetti, Suelene B N Tavares, Rodrigo A Oliveira, Aldo Solari, Ines Zulantay, Werner Apt, Patricio Diosque, Mercedes Monje Rumi, et al.

BACKGROUND: The Trypanosoma cruzi satellite DNA (satDNA) OligoC-TesT is a standardised PCR format for diagnosis of Chagas disease. The sensitivity of the test is lower for discrete typing unit (DTU) TcI than for TcII-VI and the test has not been evaluated in chronic Chagas disease patients.

METHODOLOGY/PRINCIPAL FINDINGS: We developed a new prototype of the OligoC-TesT based on kinetoplast DNA (kDNA) detection. We evaluated the satDNA ...

Differentiation between Trypanosoma cruzi and Trypanosoma rangeli using heat-shock protein 70 polymorphisms Instituut voor Tropische Geneeskunde Universiteit Antwerpen

Jorge Fraga, Aymé Fernandez-Calienes, Ana Margarita Montalvo, Ilse Maes, JC Dujardin, Gert Van der Auwera

OBJECTIVE: Differential diagnosis of infection with Trypanosoma cruzi or T. rangeli is relevant for epidemiological studies and clinical practice as both species infect humans, but only T. cruzi causes Chagas' disease. Their common antigen determinants complicate the distinction between both species, while current PCR assays used for differentiation show some drawbacks. We developed and validated a generic PCR discriminating the species by ...

In vitro evaluation of arylsubstituted imidazoles derivatives as antiprotozoal agents and docking studies on sterol 14-demethylase (CYP51) from Trypanosoma cruzi, Leishmania infantum, and Trypanosoma brucei Universiteit Antwerpen KU Leuven

Julio Alberto Rojas Vargas, America Garcia Lopez, Yulier Perez, Paul Cos, Matheus Froeyen
There is an urgent need to discover and develop new drugs to combat parasitic diseases as Chagas disease (Trypanosoma cruzi), sleeping sickness (Trypanosoma brucei), and leishmaniasis (Leishmania ssp.). These diseases are considered among the 13 most unattended diseases worldwide according to the WHO. In the present work, the synthesis of 14 arylsubstituted imidazoles and its molecular docking onto sterol 14-demethylase (CYP51) was executed. In ...

International study to evaluate PCR methods for detection of Trypanosoma cruzi DNA in blood samples from Chagas disease patients Instituut voor Tropische Geneeskunde

AG Schijman, M Bisio, L Orellana, M Sued, T Duffy, AM Mejia Jaramillo, C Cura, F Auter, V Veron, Y Qvarnstrom, et al.
BACKGROUND: A century after its discovery, Chagas disease still represents a major neglected tropical threat. Accurate diagnostics tools as well as surrogate markers of parasitological response to treatment are research priorities in the field. The purpose of this study was to evaluate the performance of PCR methods in detection of Trypanosoma cruzi DNA by an external quality evaluation. METHODOLOGY/FINDINGS: An international collaborative study ...

Phenotypic evaluation of nucleoside analogues against Trypanosoma cruzi infection : in vitro and in vivo approaches Universiteit Gent

Ludmila F. de A. Fiuza, Denise G. J. Batista, Roberson D. Girão, Fabian Hulpia, Paula Finamore-Araújo, Mustafa M. Aldfer, Ehab Kotb Elmahallawy, Harry P. De Koning, Otacílio Moreira, Serge Van Calenbergh, et al.
Chagas disease, caused by Trypanosoma cruzi (T. cruzi), is a serious public health problem. Current treatment is restricted to two drugs, benznidazole and nifurtimox, displaying serious efficacy and safety drawbacks. Nucleoside analogues represent a promising alternative as protozoans do not biosynthesize purines and rely on purine salvage from the hosts. Protozoan transporters often present different substrate specificities from mammalian ...