Polynucleotide shuffling method KU Leuven
It is a first object of the present invention to provide a method for preparing a Tile vector, being a vector, which comprises a selectable marker and a coding polynucleotide, wherein said coding polynucleotide is immediately preceded and followed by a type Ms recognition sequence, wherein said preceding and following recognition sequences are recognized by a same type lis restriction enzyme, but have an opposite orientation. More particularly, the position and orientation of said preceding and following type lis recognition sequences provides for the cleavage of said Tile vector by a ...