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Hydrochloric vs. sulphuric acid in water for Ziehl-Neelsen staining of acid-fast bacilli Instituut voor Tropische Geneeskunde

KJM Aung, P Nandi, A Hamid Salim, A Hossain, A Van Deun
SETTING: Damien Foundation Bangladesh tuberculosis (TB) control projects. OBJECTIVES: To compare 25% sulphuric acid in water (H(2)SO(4)) with hydrochloric acid in water (HCl) to differentiate acid-fast bacilli in sputum smears stained with 1% carbolfuchsin. DESIGN: For 1 year, all 158 microscopy laboratories used either H(2)SO(4) or 3%/6%/10% HCl for their routine work, alternating monthly between H(2)SO(4) and HCl. Each month a sample of five ...

Diagnostic performance of smear microscopy and incremental yield of Xpert in detection of pulmonary tuberculosis in Rwanda Instituut voor Tropische Geneeskunde

Jean Claude Semuto Ngabonziza, Willy Ssengooba, Florence Mutua, Gabriela Torrea, Augustin Dushime, Michel Gasana, Emmanuel André, Schifra Uwamungu, Alaine Umubyeyi Nyaruhirira, Dufton Mwaengo, et al.

Background: Tuberculosis control program of Rwanda is currently phasing in light emitting diode-fluorescent microscopy (LED-FM) as an alternative to Ziehl-Neelsen (ZN) smear microscopy. This, alongside the newly introduced Xpert (Cepheid, Sunnyvale, CA, USA) is expected to improve diagnosis of tuberculosis and detection of rifampicin resistance in patients at health facilities. We assessed the accuracy of smear microscopy and the incremental ...

Methylene blue is a good background stain for tuberculosis light-emitting diode fluorescence microscopy Instituut voor Tropische Geneeskunde

Armand Van Deun, KJ Aung, A Hamid Salim, Mourad Gumusboga, P Nandi, MA Hossain
SETTING: Damien Foundation Bangladesh tuberculosis (TB) control projects. OBJECTIVES: To compare blue ink, potassium permanganate and methylene blue background staining for transmitted light-emitting diode (LED) TB fluorescence microscopy (FM). DESIGN: Auramine smears made in triplicate from Ziehl-Neelsen (ZN) acid-fast bacilli (AFB) positive or negative sputum and stained with one of the background variations were read blind by LED FM. ...