Publicaties
Site-selective functionalization of nanobodies using intein-mediated protein ligation for innovative bioconjugation Universiteit Hasselt
Bioconjugation of quantum dots : review & impact on future application Universiteit Gent
Emerging site-specific bioconjugation strategies for radioimmunotracer development Vrije Universiteit Brussel
INTRODUCTION: Radioimmunotracers are a promising class of companion diagnostics for precision medicine. They are composed of an antibody-based targeting agent and a radiolabeled imaging probe. Together with the tendency towards the use of small antibody-derived fragments, the employed conjugation method is gaining increasing attention. Conventional bioconjugation methods result in heterogeneous tracer populations of which the single elements ...
Triazolinedione protein modification: from an overlooked off-target effect to a tryptophan-based bioconjugation strategy. Vlaams Instituut voor Biotechnologie Universiteit Gent
Synthesis, modification, bioconjugation of silica coated fluorescent quantum dots and their application for mycotoxin detection KU Leuven Universiteit Gent
Controlled and covalent bioconjugation of materials with site-specifically functionalized nanobodies for advanced medical diagnostics Universiteit Hasselt
Dynamic Light Scattering as a Powerful Tool for Gold Nanoparticle Bioconjugation and Biomolecular Binding Studies KU Leuven
5-Hydroxy-pyrrolone based building blocks as maleimide alternatives for protein bioconjugation and single-site multi-functionalization Universiteit Gent
Site-Selective Functionalization of Nanobodies Using Intein-Mediated Protein Ligation for Innovative Bioconjugation Vrije Universiteit Brussel
An expression strategy is presented in order to produce nanobodies modified with a clickable alkyne functionality at their C-terminus via the intein-mediated protein ligation (IPL) technique. The protocol focuses on the cytoplasmic expression and extraction of a nanobody-intein-chitin binding domain (CBD) fusion protein in E. coli SHuffle® T7 cells, in the commonly used Luria-Bertani (LB) medium. The combination of these factors results in a ...