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Alternative Routes to Induce Naive Pluripotency in Human Embryonic Stem Cells KU Leuven Universiteit Gent
Human embryonic stem cells (hESCs) closely resemble mouse epiblast stem cells exhibiting primed pluripotency unlike mouse ESCs (mESCs), which acquire a naïve pluripotent state. Efforts have been made to trigger naïve pluripotency in hESCs for subsequent unbiased lineage-specific differentiation, a common conundrum faced by primed pluripotent hESCs due to heterogeneity in gene expression existing within and between hESC lines. This required ...
The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency KU Leuven
Early during preimplantation development and in heterogeneous mouse embryonic stem cells (mESC) culture, pluripotent cells are specified towards either the primed epiblast or the primitive endoderm (PE) lineage. Canonical Wnt signaling is crucial for safeguarding naive pluripotency and embryo implantation, yet the role and relevance of canonical Wnt inhibition during early mammalian development remains unknown. Here, we demonstrate that ...
Application of small molecules favoring naive pluripotency during human embryonic stem cell derivation Universiteit Gent
Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background Universiteit Gent
Uncovering the novel role of mTORC2 subunit of the PI3K/AKT/mTORC pathway in establishing naive pluripotency in human embryonic stem cells Universiteit Gent
Comparative analysis of different culture conditions inducing naive pluripotency in human embryonic stem cells Universiteit Gent
Parallel derivation of isogenic human primed and naive induced pluripotent stem cells KU Leuven
Induced pluripotent stem cells (iPSCs) have considerably impacted human developmental biology and regenerative medicine, notably because they circumvent the use of cells from embryonic origin and offer the potential to generate patient-specific pluripotent stem cells. However, conventional reprogramming protocols produce developmentally advanced, or primed, human iPSCs (hiPSCs), restricting their use to postimplantation human development ...
Direct comparison of distinct naive pluripotent states in human embryonic stem cells KU Leuven Universiteit Gent
Until recently, human embryonic stem cells (hESCs) were shown to exist in a state of primed pluripotency, while mouse embryonic stem cells (mESCs) display a naive or primed pluripotent state. Here we show the rapid conversion of in-house-derived primed hESCs on mouse embryonic feeder layer (MEF) to a naive state within 5-6 days in naive conversion media (NCM-MEF), 6-10 days in naive human stem cell media (NHSM-MEF) and 14-20 days using the ...
Induction of Human Extraembryonic Mesoderm Cells from Naive Pluripotent Stem Cells. KU Leuven
The human extraembryonic mesoderm (EXM) is an important tissue in the postimplantation embryo which is specified before gastrulation in primates but not in rodents. EXM is mesenchymal and plays an important role in embryogenesis, including early erythropoiesis, and provides mechanical support to the developing embryo. Recently, it has been shown that self-renewing extraembryonic mesoderm cells (EXMCs) can be modeled in vitro by using human naive ...