Evaluation of a generic in vivo approach for the site-specific modification of proteins with a 'click chemistry functionalized amino acid' for the development of innovative bioactive materials Universiteit Hasselt
The site-specific modification of proteins is of great importance for the development and improvement of many biotechnological applications (e.g. biosensors). The focus of this work is the in vivo site-specific modification of proteins by means of amber suppression. As a result, a non-natural amino acid can be introduced to the protein, which can serve as a unique chemical “handle” to immobilize proteins in an oriented and covalent way, ...