Unlocking the Molecular Secrets of Pancreatic Cancer: A Multi-Omics and Live-Cell Imaging Approach to Understanding Tumor Microenvironment Crosstalk and Therapeutic Target Identification

Our team proposes an innovative project that integrates a 3D patient-derived pancreatic microtumor model with multiomics analyses to dissect molecular mechanisms of Pancreatic Ductal Adenocarcinoma (PDAC) at a single-cell, patient-specific level. A key strength of our approach is the direct link between the clinic and the laboratory, facilitated by the surgeon's unique position. This connection is crucial for accurately recreating the patient's tumor in the lab, allowing us to obtain high-quality, fresh tumor samples directly from surgeries. We aim to understand the intercommunication and reprogramming between malignant cells, stromal cells, and macrophages by applying single-cell RNA-sequencing, secretomics, and proteomics. Additionally, we will employ our AI-driven high-throughput drug screening platform to visualize live cell interactions and study cell behavior in a 3D context. This setup allows us to scrutinize the biological and therapeutic impact of identified communication pathways by testing new therapeutic strategies on our microtumor model, thereby monitoring cell-specific responses. This research could revolutionize our understanding of PDAC, paving the way for the identification of new therapeutic targets and the development of effective treatments. The synergy between the clinic

Keywords:MULTI-OMICS ANALYSIS, IN VITRO MODEL

Disciplines:Abdominal surgery