The production and validation of fluorescent nanomaterials for detection and therapy of fungi and tumors

In the present project, the goal is to generate lipid-based and polymeric nanoparticles loaded with ICG as a fluorescent and photodynamic dye. We will use peptide and antibody based approaches for the nanoparticles to reach the intended target, being either Aspergillus fumigatus, or triple negative breast cancer, specifically. Using the ICG-dye, we will use it to evaluate the biodistribution and relative targeting efficacy in murine models, using FLECT/CT imaging. Simultaneously, the ICG will be explored as a photodynamic therapy model, in order to treat both the fungal disease and tumors. We will furthermore explore the use of iron oxide nanoparticles, rather than ICG and explore the use of magnetic particle imaging (MPI) and image-based magnetic hyperthermia to monitor the biodistribution and apply local heating for therapeutic purposes. We will then combine these nanomaterials with additional agents (anti-fungal drugs; small molecules) and monitor the release of the therapeutic agent from our nanocarriers through the fluorescent or magnetic particle imaging. In the end, we will compare the therapeutic efficacy of the different nanomaterials in view of fungal and cancer therapy.