Molecular Characterization, Immunological Profiling, and Diagnostic Kit Development for Lumpy Skin Disease in Ethiopia

The first instances of lumpy skin disease (LSD) were recorded in Africa in 1929, and since then, LSD has spread to various regions, including Ethiopia, where it is considered economically significant disease. Investigations have been made on LSD outbreak samples in the country with limited information on domestic and wild life interface. As part of the investigation and diagnostic tool different methods of serological tests and antigen-targeting techniques have been used in different institutions. However, these diagnostic tools often suffer from limitations such as being time-consuming and inaccessible, particularly in developing countries. Therefore, this Ph.D. project is aimed to address these gaps by employing integrative approaches encompassing molecular and immunological characterization of LSDV, evaluation of diagnostic kits, and the development of specific diagnostic assays. The ultimate objective is to improve early detection and management of LSD through lateral flow assay (LFA) development in Ethiopia while determining the immunological correlates of protection against LSDV.