Accurate counting and sizing platform for the characterization of a broad range of nano- and microparticles

Nano and microparticles are used everywhere: in medicine, biology, cosmetics, food, etc. An accurate characterization of two of their most important properties, namely concentration and size, is crucial to assist and optimize particle synthesis, and quantify their safety and efficacy for a given intended use. Amongst the options available for particle sizing and counting, Coulter counters offer unmatched accuracy and resolution, a high flexibility regarding particle type (cells, bacteria, aggregates, drugs, …) and size (200 nm to 1.6 mm), and are robust, fast, and easy to operate. Coulter Counters measure changes in electrical resistance when single particles traverse an aperture, leading to a direct measurement of the particle volume. Unlike their competitors, Coulter Counters are therefore not dependent on particle shape, structure, or optical properties. We will exploit the many advantages of a Coulter Counter to characterize different particles beyond the current state-of-the-art, including but not limited to contrast agents, air bubbles, metallic nanoparticles, cells, and flocs.

Keywords:Particle sizing and counting, Microbubble, Coulter Counter, Resistive Pulse Sensing, Particle characterization

Disciplines:Analytical separation and detection techniques, Physical chemistry of materials, Chemistry of clusters, colloids and nanomaterials, Particle design and technology, Nanoscale characterisation