Towards B cell specific targeting in SCI: unraveling the transcriptional gene pathways underlying CD74- induced proinflammatory B cell functions (R-15809)

Following traumatic spinal cord injury (SCI), an inflammatory and autoreactive immune reaction is triggered, leading to further damage in a secondary injury phase. Recent studies have implicated B cells, a type of white blood cell, as important players in this damaging response. However, the mechanisms that trigger these pro-inflammatory B cell responses in SCI remain unknown. Our previous research has indicated that the macrophage migration inhibitory factor (MIF) and its receptor CD74 are key drivers of B cell responses after SCI. The aim of this research project is to elucidate the functional gene pathways underlying CD74-induced pro-inflammatory B cell function. The specific genes underlying CD74 signaling in B cells will be identified using transcriptome analysis. The role of these potential targets for B cell function will be studied using in vitro assays on human primary B cells. Finally, the role of these CD74-induced targets in pathology and clinical outcomes will be studied in an SCI model. This research aims to help better understand the harmful inflammatory pathways in spinal cord injuries. This knowledge will lead to the identification of novel therapeutic targets and could contribute to the development of new therapies for SCI by specifically influencing certain B cells.

Keywords:B cells, secondary inflammation, spinal cord injury

Disciplines:Adaptive immunology, Biomarker discovery, Biomarker evaluation, Analysis of next-generation sequence data