Use of stereolithographic model in tooth autotransplantation and periodontal ligament stem cells for pulp revascularization and reinnervation to increase the success rate for tooth replacement in children
Loss of permanent teeth is a particular challenge in children considering that alveolar bone growth is not yet complete and treatment approaches should adapt to both growth and developmental changes in the oral region to have the potential for long-term survival. This could be realized via autotransplantation, which is a biological approach for tooth replacement in young children. Key factor for its success is the preservation of the periodontium of the grafted tooth. However, the cut in vascular supply of the dental pulp can result in necrosis and in some cases this can lead to a loss of autotransplanted tooth. Thus the aim of this project is to compare the outcome of a novel approach using a CBCT-based surgical planning and transfer technique including a stereolithographic surgical guide and tooth replica to the outcome of a traditional autotransplantation protocol and also to explore the angiogenic and neurogenic properties of PDLSCs in order to develop a new therapeuric strategy in which topical application of specific angiogenic and neurogenic factors on the donor tooth, together with the surgical planning and stereolithographic model will improve the outcome of the autotransplantation technique.