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The use of pan-tropomyosin receptor kinase immunohistochemistry as a screening tool for the detection of neurotrophic tropomyosin-related kinase fusions : real-world data from a national multicentric retrospective study Ghent University KU Leuven University of Antwerp

Mieke R. Van Bockstal, Gabriela Beniuga, Ligia Craciun, David Creytens, Franceska Dedeurwaerdere, Philippe Delvenne, Pieter Demetter, Bart De Wiest, Koen Dewinne, Lionel Habran, et al.
Introduction: The neurotrophic tropomyosin-related kinase (NTRK) genes encode the tropomyosin receptor kinases (TRKs). Patients with solid tumors harboring an oncogenic NTRK fusion are eligible for treatment with TRK inhibitors. NTRK fusion is often associated with TRK overexpression. Pan-TRK immunohistochemistry (IHC) is used to screen for NTRK fusions, but immunoreactivity patterns are poorly defined. Methods: Data on pan-TRK immunoreactivity ...

Diagnosis of verruciform acanthotic vulvar intra-epithelial neoplasia (vaVIN) using CK17, SOX2 and GATA3 immunohistochemistry Ghent University

Eleanor Cook, Koen Van de Vijver, Carlos Parra-Herran
AimsVerruciform acanthotic vulvar intra-epithelial neoplasia (vaVIN) is an HPV-independent, p53 wild-type lesion with distinct morphology and documented risk of recurrence and cancer progression. vaVIN is rare, and prospective distinction from non-neoplastic hyperplastic lesions can be difficult. CK17, SOX2 and GATA3 immunohistochemistry has emerging value in the diagnosis of HPV-independent lesions, particularly differentiated VIN. We aimed to ...

SV40 large T-antigen and human pleural mesothelioma. Screening by polymerase chain reaction and tyramine-amplified immunohistochemistry Vrije Universiteit Brussel

Karl Dhaene, Adriaan Verhulst, E. Van Marck

DNA-like sequences of the p53 and pRB-inactivating simian virus 40 large T-antigen (SV40 LTag) have recently been found in mesotheliomas in the United States and several European countries. Nuclear expression of SV40 LTag, possibly in concert with detectable telomerase activity, could be responsible for immortalisation of (pre)malignant clones, as suggested by the mesothelioma-specific latency period. Depending on the antibody used, different ...

Interobserver variation in the assessment of immunohistochemistry expression levels in HER2-negative breast cancer : can we improve the identification of low levels of HER2 expression by adjusting the criteria? An international interobserver study Ghent University KU Leuven University of Antwerp

Ximena Baez-Navarro, Mieke R van Bockstal, Diënna Nawawi, Glenn Broeckx, Cecile Colpaert, Shusma C Doebar, Marieke C H Hogenes, Esther Koop, Kathleen Lambein, Dieter J E Peeters, et al.
The classification of human epidermal growth factor receptor 2 (HER2) expression is optimized to detect HER2-amplified breast cancer (BC). However, novel HER2-targeting agents are also effective for BCs with low levels of HER2. This raises the question whether the current guidelines for HER2 testing are sufficiently reproducible to identify HER2-low BC. The aim of this multicenter interna-tional study was to assess the interobserver agreement of ...

Status of Programmed Death Ligand 1 (PD-L1) by Immunohistochemistry and Scoring Algorithms KU Leuven

Thomas Gevaert
The detection of the Programmed Death Ligand 1 (PD-L1) protein by immunohistochemistry is currently the only approved test predictive of response to drugs targeting the PD1/PDL1 axis. The role of this test is debated since several reagents have been used as companion diagnostics for different drugs on diverse immunostaining platforms. In addition, different scoring systems for PD-L1 immunohistochemistry have been applied in the registration ...

Validated programmed cell death ligand 1 immunohistochemistry assays (E1L3N and SP142) reveal similar immune cell staining patterns in melanoma when using the same sensitive detection system KU Leuven Vrije Universiteit Brussel University of Antwerp

Isabelle Vanden Bempt
AIMS: Tumour cell and/or immune cell programmed cell death ligand 1 (PD-L1) expression is considered as a potential biomarker for anti-PD1 and anti-PD-L1 immunotherapy. Currently, different PD-L1 assays are used. This study aims to compare the staining patterns of two PD-L1 antibody clones in melanoma metastases and correlate them with PD-L1 mRNA expression. METHODS AND RESULTS: The immunohistochemistry assays were optimized and validated ...

Dietary supplementation of microencapsulated sodium butyrate in healthy horses: effect on gut histology and immunohistochemistry parameters Research Institute for Agriculture, Fisheries and Food Ghent University

Wendy Wambacq, David van Doorn, P Rovers-Paap, Richard Ducatelle, Lieven Vlaminck, Marta Lourenço Ribeiro Alves, Myriam Hesta
Background: As colic and intestinal disorders are a major concern in horses, the aim of the present study was to investigate the effect of dietary supplementation of butyrate, known to have a diverse array of beneficial effects on intestinal health. The effect of micro-encapsulated sodium butyrate supplementation on gut histology and immunohistochemistry parameters was studied in 14 healthy warmblood horses destined for slaughter in two separate ...

Methods to assess autophagy in situ: transmission electron microscopy versus immunohistochemistry University of Antwerp

Wim Martinet, Jean-Pierre Timmermans, Guido De Meyer
Autophagy is a well-conserved lysosomal degradation pathway that plays a major role in both oncogenesis and tumor progression. Transmission electron microscopy (TEM) as well as immunohistochemistry are indispensable tools for the evaluation of autophagy in situ. Here, we describe an optimized protocol for the study of autophagic vacuoles by TEM and elaborate on the immunohistochemical detection of microtubule-associated protein 1 light chain ...