Infrastructure
Platform for Next Generation Screening in Quantitative Biology & Drug Discovery (NEXTGEBQBIO) KU Leuven
In this proposal we outline a state-of-the-art platform that would be among the first in Europe to combine the best in automated microscopy with advanced cell culture robotics capable of automated isolation, expansion and differentiation of mammalian and human cells, including iPSC. The platform will be unique in Flanders and will allow running quantitative image-based screens as well as screens for disease phenotypes using conventional cell lines but more importantly also on human stem cells, which provide the closest mimic of human pathology currently available. Not only will this prove ...
A multimodal non-linear optical platform for 2l3-photon excitation and 2ndl3rd harmonic imaging deep inside living tissues and animals (Multimodal NLO imaging platform) Ghent University
The infrastructure is an advanced and multimodal non-linear optical (NLO) imaging platform for imaging of cells in their native, intact, 3-dimensional (3D) environment over extended periods of time using 2-photon (2P) and 3-photon (3P) excitation principles while providing extra structural context and label-free information by exploiting 2nd and 3rd harmonic signals generated by endogenous molecules, nanomedicines or pollutants.
The platform with its dual 2P|3P pulsed laser configuration:
* allows to reach deeper into tissue structures, enabling non-invasive in-vivo optical ...
Fluorescence lifetime imaging microscope (FLIM) Ghent University
Fluorescence lifetime imaging microscopy (FLIM) is a tool for studying biological processes in a live, multiparameter and quantitative manner. Modern FLIM confocal microscopes equipped with respective hardware and software enable rapid and high-quality 3D analysis of cell and tissue oxygenation, metabolism, cell proliferation and differentiation and their response to therapy and infection. FLIM systems already meet the requirements to work with patient-derived tumor xenografts, organoids and advanced 3D cell- and tissue-based models, but are open to the next generation imaging ...