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Project

Development of an interdisciplinary platform for spatial multi-omic analysis of single cells

Single-cell sequencing is revolutionizing our understanding of the extent, nature, and biology of cellular diversity in an organism. However, since the concerted action of individual cells executes most of our organ functions in a spatially organized context, it is also paramount to investigate cells in their natural habitat. This is not only important to understand the architecture and function of an organ, but also to study how (subpopulations of) cells are perturbed in disease via assembly of aberrant cell niches or altered cell-cell communication. Despite this need, current technologies for spatial analyses of cells in situ are limited in their experimental and analytical approaches. With our interdisciplinary network, we will establish novel single-cell in situ analysis protocols, fluidic-enhanced imaging platforms, and algorithms that enable (multi)omic readouts at subcellular resolution in single cells in their histological tissue context in high throughput.
Date:1 Oct 2019 →  30 Sep 2023
Keywords:Spatial multi-omics, Single-cell multi-omics, Expansion microscopy, Image analysis, Cell Heterogeneity
Disciplines:Genetics, Single-cell data analysis, Histology, Data visualisation and high-throughput image analysis, Photochemistry, Bio-organic chemistry, Organic chemical synthesis, Spectroscopic methods