Prelimbic cortical injections of a GABA agonist and antagonist

Ondertitel:in vivo quantification of the effect in the rat brain using $[^{18}F]$ FDG microPET

Purpose We evaluated the glucose metabolism after microinjections of a GABAA antagonist, bicuculline, and a GABAA agonist, muscimol, in the rat prelimbic cortex (PL) by small animal positron emission tomography (μPET). Procedures Following a microinjection of either 0.5 μl bicuculline (0.1 mg/ml), muscimol (1 mg/ml), or saline in the left PL of 11 healthy male Sprague Dawley rats (250275 g), 2-deoxy-2-[18F]fluoro-β-d-glucose ([18F]FDG) PET images were acquired. Volume-of-interest (VOI)-based analysis and voxel-based statistical parametric mapping were performed (n = 9). Results VOI-based analysis revealed significantly different [18F]FDG uptake following bicuculline versus muscimol in PL (p < 0.001), infralimbic cortex (p < 0.01), and cingulate cortex (p < 0.01). Voxel-based analysis showed bicuculline induced widespread significant hypermetabolism throughout the brain while muscimol induced significant localized hypometabolism. Conclusions Here, we visualize functional GABAA-mediated correlations of the PL following pharmacological stimulation. This could serve as a reference and shed light on the working and focality of other stimulation paradigms targeting this region.

Jaar van publicatie:2015

Trefwoorden:A1 Journal article

BOF-keylabel:ja

BOF-publication weight:1

CSS-citation score:1

Authors from:Higher Education

Toegankelijkheid:Closed