Publicaties

Biology is becoming increasingly data-dependent, and computation is in the course of becoming indispensable for many research avenues. The field of phage lysins is not an exception to this new paradigm. But, to make the most of it, meaningful, easily accessible data is a major requirement. To this end, PhaLP was created four years ago as a centralized resource of phage lysin sequences and associated metadata intended to empower lysin researchers ...

Antimicrobiële resistentie vormt een kritische wereldwijde gezondheidscrisis waardoor traditionele antibiotica grotendeels ineffectief worden en er dus dringend naar nieuwe oplossingen gezocht moet worden. Geïnspireerd door de natuur onderzoeken wetenschappers bacteriofagen als veelbelovende bron van nieuwe antibiotica. Deze bacteriofagen zijn virussen die bacteriën infecteren en vernietigen. Zij produceren daarvoor faaglysines, enzymen die snel ...

Designer cellulosomes (DCs) are precisely engineered multi-enzyme complexes aimed at lignocellulose saccharification. Achieving spontaneous designer cellulosome display on yeast cell surfaces has been a long-term objective to enhance consolidated bioprocesses with concurrent ethanol production. A “self-assembly” approach involves simultaneous scaffoldin display and docking enzyme secretion. However, challenges arise from the size and complexity ...

SPAED is an accessible tool for the accurate segmentation of protein domains that leverages information contained in the predicted aligned error (PAE) matrix obtained from AlphaFold to better identify domain-linker boundaries and detect terminal disordered regions. On a dataset of 376 bacteriophage endolysins (proteins that degrade the bacterial cell wall), SPAED achieves a mean intersect-over-union score of 96% and a domain-boundary-distance ...

Lysins are a promising class of bacteriophage-derived, enzyme-based antibacterials with a new mode-of-action based on active peptidoglycan degradation. While initiallyconsidered for human healthcare only, their potential market expands to each sector where bacteria are undesired. This includes the fermentation industry which sufferssignificant yield and profit losses from recurrent bacterial contaminations. Additionally, their modular nature and ...

Phage lysins, bacteriophage-encoded enzymes tasked with degrading their host’s cell wall, are increasingly investigated and engineered as novel antibacterials across diverse applications. Their rapid action, tuneable specificity, and low likelihood of resistance development make them particularly interesting. Despite numerous application-focused lysin studies, the art of their recombinant production remains relatively undiscussed. Here, we ...

With uncontrolled antimicrobial resistance on the rise, developing new and improved antibacterial strategies is imperative. Today, bacteriophage-encoded lysins are a highly promising new class of antibacterials that respond to this call. These bacteriolytic enzymes are featured by their rapid mode-of-action based on active peptidoglycan degradation. Additionally, due to their modular nature and wide abundance, they are ideal candidates for ...

With uncontrolled antimicrobial resistance on the rise, developing new and improved antimicrobial strategies is imperative. Today, bacteriophage-derived lysins are a highly promising new class of antibacterials that respond to this call. These bacteriolytic enzymes or enzybiotics are featured by their rapid mode-of-action based on active peptidoglycan degradation. Additionally, due to their modular nature and wide abundance, they are ideal ...