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Biocompatible Label-Free Detection of Carbon Black Particles by Femtosecond Pulsed Laser Microscopy Universiteit Hasselt KU Leuven
Although adverse health effects of carbon black (CB) exposure are generally accepted, a direct, label-free approach for detecting CB particles in fluids and at the cellular level is still lacking. Here, we report nonincandescence related white-light (WL) generation by dry and suspended carbon black particles under illumination with femtosecond (fs) pulsed near-infrared light as a powerful tool for the detection of these carbonaceous materials. ...
Label-free Protein Detection Based on the Heat-Transfer Method-A Case Study with the Peanut Allergen Ara h 1 and Aptamer-Based Synthetic Receptors Universiteit Hasselt KU Leuven
Aptamers are an emerging class of molecules that, because of the development of the systematic 'evolution of ligands by exponential enrichment (SELEX) proceSs, can recognize Virtually every target ranging from ions, to proteins, and even whole Cells. Although there are niany techniques capable of detecting template molectjles with aptamer-based systems with high specificity and selectivity, they lack the possibility of integrating them into a ...
Label-free surface enhanced Raman spectroscopy for cancer detection Universiteit Gent
Nanocrystalline diamond impedimetric aptasensor for the label-free detection of human IgE Universiteit Hasselt KU Leuven
Like antibodies, aptamers are highly valuable as bioreceptor molecules for protein biomarkers because of their excellent selectivity, specificity and stability. The integration of aptamers with semiconducting materials offers great potential for the development of reliable aptasensors. In this paper we present an aptamer-based impedimetric biosensor using a nanocrystalline diamond (NCD) film as a working electrode for the direct and label-free ...
Label-free detection and size estimation of combustion-derived carbonaceous particles in a microfluidic approach KU Leuven
Detection and size estimation of combustion-derived carbonaceous particles (CDCPs) are important to understand their toxicity. Size determination of individual nano- and microparticles (NMPs) based on scattered light is a straightforward method. However, detection and sizing of CDCPs in biological samples based on scattering alone are not possible due to the compositional heterogeneity of NMPs present in biological samples. Label-free ...
Rapid and label-free optical detection of individual carbon air pollutant nanoparticulates in biomedical samples KU Leuven
Carbonaceous particle exposure and air pollution in general lead to a multitude of adverse human health effects and pose multiple challenges in terms of exposure, risk and safety assessment. Highly desirable for fast screening are label-free approaches for detecting these particle types in biological or medical context. We report a powerful approach for detecting carbonaceous particles using photothermal pump-probe microscopy, which directly ...
Label-free detection of uptake, accumulation, and translocation of diesel exhaust particles in ex vivo perfused human placenta Universiteit Hasselt KU Leuven
BackgroundPregnant women and developing fetuses comprise a particularly vulnerable population as multiple studies have shown associations between prenatal air pollution exposure and adverse pregnancy outcomes. However, the mechanisms underlying the observed developmental toxicity are mostly unknown, in particular, if pollution particles can cross the human placenta to reach the fetal circulation.ResultsHere, we investigated the accumulation and ...
INTEGRATION OF HEAT-TRANSFER RESISTANCE MEASUREMENTS ONTO A DIGITAL MICROFLUIDIC PLATFORM TOWARDS THE MINIATURIZED AND AUTOMATED LABEL-FREE DETECTION OF BIOMOLECULAR INTERACTIONS Universiteit Hasselt KU Leuven
In this paper the successful integration of heat-transfer resistance measurements with a digital microfluidic chip is shown. The integrated miniaturized platform allows the automated label-free detection of biomolecular interactions. To immobilize biomolecules on the hydrophobic chip surface, hydrophilic gold sensing patches are created by means of a recently described dry lift-off technique that leaves the chip surface unaffected. DNA melting ...
Development of an impedimetric sensor for the label-free detection of the amino acid sarcosine with molecularly imprinted polymer receptors KU Leuven
© 2017 Elsevier B.V. In this article we report on the development and optimization of a biomimetic sensor for the label-free detection of the amino acid sarcosine, a molecule which is seen as a biomarker for prostate cancer. The recognition elements were sarcosine-imprinted poly-aminothiophenol (p-ATP) layers deposited by electro-polymerization onto screen-printed gold electrodes and, for comparison, onto carbon electrodes covered first with a ...