Researcher

Diana Campillo Davó

Research Expertise:I specialize in genetic engineering of immune cells using non-viral methods, including but not limited to messenger RNA electroporation and CRISPR-Cas system. My lines of research mainly focus on developing novel therapeutic cellular products based on T cells engineered with antigen-specific T-cell receptors (TCRs) and chimeric antigen receptors (CARs) for targeting hematological malignancies such as acute myeloid leukemia. For this, I have broad expertise in cellular and molecular research techniques (RNA synthesis, plasmid design, etc), multiparametric flow cytometry, in vitro functional analyses, and analysis of raw data and statistical analysis with relevant software.

Keywords:CANCER RESEARCH, GENE THERAPY, IMMUNOLOGY, IMMUNOTHERAPY, CANCER IMMUNOTHERAPY, Medicine

Disciplines:Hematology, Immunology, Molecular and cell biology, Oncology, Applied immunology, Cancer therapy

Research techniques:Multicolor flow cytometry, Cell culture, ELISA, ELISpot, T-cell functional assays (activation markers, cytokine secretion, cytotoxicity assays, etc.), Transfection, Electroporation, CRISPR knock-out, siRNA assays, Molecular biology (plasmid amplification, restriction assays, electrophoresis, DNA and RNA extraction, in vitro RNA synthesis, etc.), PCR, RT-qPCR, DNA plasmid design, CAR and TCR design.

Users of research expertise:Basic, translational and clinical researchers, biotech and pharmaceutical companies, research funders.