BIO-FET-BASED PEPTIDE SEQUENCING METHOD AND DEVICE

The present disclosure relates to single-peptide sequencing. A method for sequencing peptides is provided that includes attaching a base end of a peptide to a channel region surface of a biosensor field effect transistor (bio-FET) and obtaining a first measurement of a parameter of the bio-FET, the parameter depending on the bio-FET's threshold voltage. The method further includes providing first molecular probes into the electrolyte gate, each first molecular probe being linked to a respective charge tag, and obtaining a second measurement of the parameter. Then, the method includes determining whether a shift of the parameter occurred between the first and second measurements, which indicates that a first molecular probe attached to an amino acid at a free end of the peptide. If the shift of the parameter occurred, the method includes determining a type of the amino acid based on a type of the first molecular probe.

Octrooi-publicatienummer: US20240218441

Bron: USPTO

Jaar van publicatie: 2024

Status: Aangevraagd

Technologiedomeinen: undefined