Method for sequencing a polynucleotide using a biofet KU Leuven
A method for sequencing a template polynucleotide, comprising the steps of:a) providing a sensor (1) comprising:- an active region comprising a source region, a drain region, and a channel region (2),- a dielectric region (3) on the channel region (2),- a polymerase (4) coupled to the dielectric region (3), the polymerase having an active site (5), the polymerase (4) being separated from the dielectric region (3) by a gap,- one or more sensitizing means,- a fluidic gate region (8) to which the polymerase (4) is exposed,- a template polynucleotide bound to a primer, the template polynucleotide ...