Projects
White light laser-based confocal fluorescence lifetime imaging microscope for multi-parameter 3D live imaging Ghent University
Fluorescence lifetime imaging microscopy (FLIM) is anemerging tool for studying biological processes in live,multi-parameter and quantitative manner. Modern FLIM-compatible confocal microscopes equipped with respectivehardware and software enable for fast and high-quality 3Danalysis of cell and tissue oxygenation, metabolism, cellproliferation and differentiation, their response to therapyand infection. While already able addressing ...
A microscope for fast and super-resolution fluorescence imaging KU Leuven
Fluorescence microscopy is an indispensable tool in biological sciences. Over the years, the need for faster imaging systems increased with the growing number of three-dimensional samples. The goal of this project is the construction of a fast and sensitive line-scan confocal microscope. The proposed instrument will acquire images at least an order of magnitude faster than point-scan confocal microscopes without compromising sensitivity. The ...
A compact microscope for long term fluorescence imaging of live cells KU Leuven
Multimodal Coherent Raman Scattering /Fluorescence Lifetime Imaging Microscopy system KU Leuven
Advanced multimodal microscopy system for super-multiplexed
high resolution subcellular imaging of metabolic processes and biomolecules in live cells, organoids, cancer tissues and organisms based on Coherent Raman Scattering (CRS) vibrational imaging and Fluorescence Lifetime Imaging Microscopy (FLIM).
Correlating spectroscopic properties of fluorescent proteins (FPs) tot heir crystal structure: towards the rational design of FPs for superresolution microscopy. KU Leuven
Exploring excited state structural dynamics of fluorescent proteins by using X-ray free electron laser (XFEL) towards advancement of cutting‐edge microscopy KU Leuven
The development of novel quenched fluorescent activity-based probes and their application in the visualization of serine protease activity in living cells by microscopy and flow cytometry KU Leuven
Proteases are enzymes that cut proteins. Although some play a role in food digestion, most are involved in the regulation of specific biological processeses in the body. Aberrant protease activity contributes to the development of diseases, and proteases can therefore be used as drug targets and diagnostic markers. For example, some tumor types display an increased protease activity. If we were able to localize a particular protease and show ...
Doctoral Network for a Shared Excellence of Fluorescent Lifetime Imaging Microscopy in Biomedical Applications Ghent University
The flIMAGIN3D project will create a research training network on the biomedical applications of 3D fluorescent lifetime imaging microscopy (FLIM). The project aims to recruit 10 doctoral candidates for the creation of robust, user friendly, innovative FLIM-based platforms for a broad range of biomedical applications. The training network will cover the essential theoretical foundation of FLIM and, through the individual projects, address the ...